Composite

Part:BBa_K2686006:Experience

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-10-08)

The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, for the 60-mer and that sfGFP binds to the encapsulin.


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UNIQe44a9890e54b6d60-partinfo-00000001-QINU UNIQe44a9890e54b6d60-partinfo-00000002-QINU